Legrand C, Bour J M, Jacob C, Capiaumont J, Martial A, Marc A, Wudtke M, Kretzmer G, Demangel C, Duval D
Laboratoire de Biochimie Médicale, Faculté de Médecine et IBN, Nancy, France.
J Biotechnol. 1992 Sep;25(3):231-43. doi: 10.1016/0168-1656(92)90158-6.
One significant problem in monitoring a culture's evolution is to assess change in cell viability. We have demonstrated that LDH release could be a good indicator of cellular damage of many cell lines, especially during shear stress or sonication. Moreover, we have found a significant correlation between the number of dead cells, determined by Trypan Blue staining, and LDH activity measurements in the supernatant of hybridoma strains, whatever the culture conditions. We have also shown that when viability is still near 100% no LDH is released even at high cell concentrations. Therefore, LDH should serve as a potential marker of cell injury and death.
监测培养物进化过程中的一个重大问题是评估细胞活力的变化。我们已经证明，乳酸脱氢酶（LDH）释放可能是许多细胞系细胞损伤的良好指标，尤其是在剪切应力或超声处理期间。此外，我们发现，无论培养条件如何，通过台盼蓝染色确定的死细胞数量与杂交瘤菌株上清液中的LDH活性测量值之间存在显著相关性。我们还表明，当活力仍接近100%时，即使在高细胞浓度下也不会释放LDH。因此，LDH应作为细胞损伤和死亡的潜在标志物。
