Matsuyama K, Matsumoto H, Matsuda A, Sugiura H, Komatsu K, Ichikawa S
Shiraoi Pharmaceutical Plant, Asahi Chemical Industry Co., Ltd., Hokkaido, Japan.
Biosci Biotechnol Biochem. 1992 Sep;56(9):1410-2. doi: 10.1271/bbb.56.1410.
Acetyl CoA: deacetylcephalosporin C O-acetyltransferase, which catalyzes the final step of the biosynthetic pathway to cephalosporin C, was stabilized by a buffer solution containing 7-aminocephalosporanic acid and purified over 1300-fold from Acremonium chrysogenum. The purified enzyme has a molecular weight of 55,000 as measured by gel filtration. SDS-polyacrylamide gel electrophoresis showed two subunit bands corresponding to molecular weights of 27,000 and 14,000. The enzyme has an isoelectoric point at pH 4.0 and optimum activity at pH 7.5.
乙酰辅酶A：去乙酰头孢菌素C O - 乙酰转移酶催化头孢菌素C生物合成途径的最后一步反应，该酶可被含有7 - 氨基头孢烷酸的缓冲溶液稳定，并从产黄顶头孢霉中纯化了1300多倍。通过凝胶过滤测得纯化后的酶分子量为55,000。SDS - 聚丙烯酰胺凝胶电泳显示有两条亚基带，分子量分别为27,000和14,000。该酶的等电点为pH 4.0，最适活性pH为7.5。
