Matsuda A, Sugiura H, Matsuyama K, Matsumoto H, Ichikawa S, Komatsu K
Pharmaceutical Research and Development Department, Asahi Chemical Industry Co., Ltd., Fuji, Japan.
Biochem Biophys Res Commun. 1992 Feb 14;182(3):995-1001. doi: 10.1016/0006-291x(92)91830-j.
Acetyl CoA: deacetylcephalosporin C o-acetyltransferase(DCPC-ATF) catalyses the final step in the biosynthesis of cephalosporin C, the conversion of deacetylcephalosporin C to cephalosporin C. A cDNA encoding DCPC-ATF has been isolated from a cDNA library of a cephalosporin C producing fungus Acremonium chrysogenum using oligonucleotide probes based on N-terminal amino acid sequences of the enzyme. The cDNA contains a single large open reading frame for a putative precursor consisting of 12 amino acid(AA) leader peptide of unknown function, 274 AA large subunit and 126 AA small subunit at the carboxyl end. The cDNA was expressed in yeast exhibiting a functional DCPC-ATF activity. It was also indicated that the leader peptide was not essential for expression of the enzyme activity. The primary structure of DCPC-ATF shows significant homology with those of acetyl CoA: homoserine o-acetyltransferase in Saccharomyces cerevisiae and Ascobolas immersus.
乙酰辅酶A：去乙酰头孢菌素C O-乙酰基转移酶（DCPC-ATF）催化头孢菌素C生物合成的最后一步，即将去乙酰头孢菌素C转化为头孢菌素C。基于该酶的N端氨基酸序列，使用寡核苷酸探针从头孢菌素C产生菌产黄顶头孢霉的cDNA文库中分离出了编码DCPC-ATF的cDNA。该cDNA包含一个单一的大开放阅读框，用于一个假定的前体，该前体由一个功能未知的12个氨基酸（AA）的前导肽、一个274个AA的大亚基和羧基端一个126个AA的小亚基组成。该cDNA在酵母中表达，表现出功能性的DCPC-ATF活性。还表明前导肽对于该酶活性的表达不是必需的。DCPC-ATF的一级结构与酿酒酵母和沉浸曲霉中的乙酰辅酶A：高丝氨酸O-乙酰基转移酶的一级结构具有显著的同源性。
