Golovleva L A, Zaborina O, Pertsova R, Baskunov B, Schurukhin Y, Kuzmin S
Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences, Pushchino, Moscow Region, Russia.
Biodegradation. 1991;2(3):201-8. doi: 10.1007/BF00124494.
The strain Streptomyces rochei 303 (VKM Ac-1284D) is capable of utilizing 2-chloro-, 2,4-, 2,6-dichloro- and 2,4,6-trichlorophenols as the sole source of carbon. Its resting cells completely dechlorinated and degraded 2-, 3-chloro-; 2,4-, 2,6-, 2,3-, 2,5-, 3,4-, 3,5-dichloro-; 2,4-, 2,6-dibromo-; 2,4,6-, 2,4,5-, 2,3,4-, 2,3,5-, 2,3,6-trichlorophenols; 2,3,5,6-tetrachloro- and pentachlorophenol. During chlorophenol degradation, a stoichiometric amount of chloride ions was released and chlorohydroquinols were formed as intermediates. In cell-free extracts of S. rochei, the activity of hydroxyquinol 1,2-dioxygenase was found. The enzyme was induced with chlorophenols. Of all so far described strains degrading polychlorophenols, S. rochei 303 utilized a wider range of chlorinated phenols as the sole sourse of carbon and energy.
罗氏链霉菌303菌株（VKM Ac-1284D）能够将2-氯苯酚、2,4-二氯苯酚、2,6-二氯苯酚和2,4,6-三氯苯酚作为唯一碳源加以利用。其静息细胞能够完全脱氯并降解2-氯苯酚、3-氯苯酚、2,4-二氯苯酚、2,6-二氯苯酚、2,3-二氯苯酚、2,5-二氯苯酚、3,4-二氯苯酚、3,5-二氯苯酚、2,4-二溴苯酚、2,6-二溴苯酚、2,4,6-三氯苯酚、2,4,5-三氯苯酚、2,3,4-三氯苯酚、2,3,5-三氯苯酚、2,3,6-三氯苯酚、2,3,5,6-四氯苯酚和五氯苯酚。在氯酚降解过程中，会按化学计量释放出氯离子，并形成氯代对苯二酚作为中间产物。在罗氏链霉菌的无细胞提取物中，发现了羟基对苯二酚1,2-双加氧酶的活性。该酶可由氯酚诱导产生。在所有目前已描述的降解多氯苯酚的菌株中，罗氏链霉菌303能利用范围更广的氯代苯酚作为唯一的碳源和能源。
