Sze I S, Dagley S
J Bacteriol. 1984 Jul;159(1):353-9. doi: 10.1128/jb.159.1.353-359.1984.
Salicylate hydroxylase (salicylate 1-monooxygenase, EC 1.14.13.1) was purified from the soil yeast Trichosporon cutaneum. The enzyme contained flavin adenine dinucleotide and was monomeric, with a molecular weight of 45,300. In addition to salicylate, the four isomeric dihydroxybenzoates having one hydroxyl adjacent to carboxyl in the benzene nucleus were oxidatively decarboxylated without formation of hydrogen peroxide. One of these isomers, gentisate, was rapidly oxidized to hydroxyquinol by the enzyme but did not serve as an effective single carbon source for T. cutaneum; however, when growing with salicylate, cells also readily utilized gentisate for growth. Hydroxyquinol 1,2-dioxygenase (EC 1.13.11....) is a newly investigated enzyme which was purified from T. cutaneum grown with 4-hydroxybenzoate. The enzyme was red, contained ferric iron, and was specific for hydroxyquinol; catechol and pyrogallol were oxidized at less than 1% of the rate for hydroxyquinol, and no activity could be detected against seven other catechols. The enzyme was composed of two nonidentical subunits having molecular weights of 39,600 and 38,200 and was apparently dimeric.
水杨酸羟化酶（水杨酸1-单加氧酶，EC 1.14.13.1）是从土壤酵母皮状丝孢酵母中纯化得到的。该酶含有黄素腺嘌呤二核苷酸，为单体，分子量为45,300。除水杨酸外，苯环上羧基邻位带有一个羟基的四种异构二羟基苯甲酸可被氧化脱羧，且不产生过氧化氢。其中一种异构体龙胆酸可被该酶迅速氧化为羟基对苯二酚，但它并不是皮状丝孢酵母有效的单一碳源；然而，当与水杨酸一起生长时，细胞也能很容易地利用龙胆酸进行生长。羟基对苯二羟基对苯二酚1,2-双加氧酶（EC 1.13.11....）是一种新研究的酶，它是从以4-羟基苯甲酸生长的皮状丝孢酵母中纯化得到的。该酶呈红色，含有三价铁，对羟基对苯二酚具有特异性；儿茶酚和连苯三酚的氧化速率不到羟基对苯二酚的1%，并且未检测到对其他七种儿茶酚的活性。该酶由两个分子量分别为三万九千六百和三万八千二百的不同亚基组成，显然为二聚体。
