CAMERON D A
J Biophys Biochem Cytol. 1961 Mar;9(3):583-95. doi: 10.1083/jcb.9.3.583.
The appearance of osteoblasts after fixation with OsO(4) is described in this paper. They have the basic structures found in other types of cells. The most striking feature is the array of rough-surfaced membranes of the endoplasmic reticulum; this feature is in keeping with the osteoblast's function of producing collagen as the bone grows. The sacs formed by these membranes probably represent the protein-containing granules described by other workers using the light microscope. They contain fine fibrillary material, and similar fibrils are to be found free in the cytoplasm. These fibrils could be tropocollagen units, although fibrils recognizable as collagen by their structure are found only outside the cell. The arrangement of the cell organelles does not seem to be related to the formation of collagen, but correlation of the fine structures of the cells with the histochemical and cytochemical findings in these cells reported by other workers leaves no doubt that they are directly concerned in the production of the organic matrix. It has not been possible to show that osteoblasts influence the passage of calcium or phosphate ions from the blood to the bone matrix.
本文描述了经四氧化锇固定后成骨细胞的外观。它们具有其他类型细胞所具有的基本结构。最显著的特征是内质网的糙面膜排列；这一特征与成骨细胞在骨骼生长时产生胶原蛋白的功能相符。这些膜形成的囊可能代表其他研究者用光学显微镜描述的含蛋白质颗粒。它们含有纤细的纤维状物质，类似的纤维在细胞质中也能自由找到。这些纤维可能是原胶原蛋白单位，尽管只有在细胞外才能发现结构上可识别为胶原蛋白的纤维。细胞器的排列似乎与胶原蛋白的形成无关，但将这些细胞的精细结构与其他研究者报道的这些细胞的组织化学和细胞化学结果进行关联后，毫无疑问它们直接参与了有机基质的产生。目前还无法证明成骨细胞会影响钙离子或磷酸根离子从血液进入骨基质的过程。
