生产和分离阿霉素A的改进工艺以及在10000升发酵罐中的大规模发酵。
Improved processes for the production and isolation of dynemicin A and large-scale fermentation in a 10,000-liter fermentor.
作者信息
Lam K S, Titus J A, Dabrah T T, Kimball D L, Veitch J M, Gustavson D R, Compton B J, Matson J A, Forenza S, Ross J
机构信息
Bristol-Myers Squibb Company, Pharmaceutical Research Institute, Wallingford, CT 06492.
出版信息
J Ind Microbiol. 1992 Nov;11(1):7-12. doi: 10.1007/BF01583725.
Supplementing the culture of Micromonospora chersina sp. nov. No. M956-1 with NaI (0.5 mg/l) enhanced the production of dynemicin A by 35-fold in shake flask culture. Homogeneous dynemicin A was obtained from the whole broth extract by Dicalite chromatography, Sephadex LH-20 chromatography and vacuum liquid chromatography. Gram quantities of dynemicin A were obtained from the fermentation of M. chersina sp. nov. No. M956-1 in a 10,000-liter fermentor.
在新种嗜热小单孢菌M956 - 1号菌株的培养中添加碘化钠（0.5毫克/升），可使摇瓶培养中达内霉素A的产量提高35倍。通过硅藻土色谱法、葡聚糖凝胶LH - 20色谱法和真空液相色谱法从全发酵液提取物中获得了达内霉素A纯品。通过在10000升发酵罐中对新种嗜热小单孢菌M956 - 1号菌株进行发酵，获得了克级量的达内霉素A。
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