Gerstner J A, Cramer S M
Isermann Department of Chemical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180-3590.
Biotechnol Prog. 1992 Nov-Dec;8(6):540-5. doi: 10.1021/bp00018a011.
Protamine was investigated for its utility as a protein displacer in cation-exchange systems. Although the protamine solution contained several variants of the molecule, the high affinity of all of the components in this heterogeneous biopolymer enabled it to act as an efficient protein displacer. To facilitate parameter estimation of the protamine, a preliminary purification was carried out by preparative elution chromatography. Chromatographic parameters of both the feed proteins and protamine displacer were obtained for use in a multicomponent steric mass action ion-exchange displacement model. Model simulations were compared to displacement results under both moderate and intense induced salt gradient conditions. In both cases, excellent agreement was obtained between the displacement experiments and theoretical predictions. In addition, these studies serve to dramatize the importance of induced salt gradients in ion-exchange displacement systems.
对鱼精蛋白作为阳离子交换系统中蛋白质置换剂的效用进行了研究。尽管鱼精蛋白溶液包含该分子的几种变体，但这种异质生物聚合物中所有成分的高亲和力使其能够作为一种有效的蛋白质置换剂。为便于对鱼精蛋白进行参数估计，通过制备性洗脱色谱法进行了初步纯化。获得了进料蛋白质和鱼精蛋白置换剂的色谱参数，用于多组分空间质量作用离子交换置换模型。将模型模拟结果与中度和强烈诱导盐梯度条件下的置换结果进行了比较。在这两种情况下，置换实验与理论预测之间都取得了极好的一致性。此外，这些研究凸显了诱导盐梯度在离子交换置换系统中的重要性。
