Verma V, Qazi G N, Parshad R
Genetic Engineering Unit, Regional Research Laboratory, Jammu Tawi, India.
J Biotechnol. 1992 Nov;26(2-3):327-30. doi: 10.1016/0168-1656(92)90016-3.
Intergeneric protoplast fusion between 2,5-diketo-gluconic acid producing Gluconobacter oxydans (ATCC 9937) and a mutant strain of Corynebacterium species (ATCC 31090), capable of reducing 2,5-diketo-gluconic acid to 2-keto-L-gulonic acid, a penultimate step in vitamin C production) resulted in viable recombinants. Some of the fusion products exhibited the capacity to convert D-glucose to 2-keto-L-gulonic acid, but the conversion rate is low.
能够产生2,5-二酮葡萄糖酸的氧化葡萄糖酸杆菌（ATCC 9937）与一株能够将2,5-二酮葡萄糖酸还原为2-酮基-L-古龙酸（维生素C生产的倒数第二步）的棒状杆菌属突变菌株（ATCC 31090）之间的属间原生质体融合产生了有活力的重组体。一些融合产物表现出将D-葡萄糖转化为2-酮基-L-古龙酸的能力，但转化率较低。
