Goergen J L, Marc A, Engasser J M
Institut National Polytechnique de Lorraine, Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC-ENSAIA, Nancy, France.
Cytotechnology. 1992;10(2):147-55. doi: 10.1007/BF00570891.
For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumption rate, but a 70% higher antibody production rate in continuous than in batch culture. Much higher metabolic rates were also measured during the initial lag phase of the batch culture. When representing the variation of the specific antibody production rate as a function of the specific growth rate, there was a positive association between growth and antibody production in the batch culture, but a negative association during the transient phase of the continuous culture. The kinetic differences between cellular metabolism in batch and continuous cultures may be the result of modifications in the physiology and metabolism of cells which, in continuous cultures, were extensively exposed to glucose limitations.
对于小鼠杂交瘤细胞系VO 208，在分批培养和连续培养中比较了其生长动力学、葡萄糖和谷氨酰胺消耗以及乳酸、氨和抗体的产生情况。在给定的比生长速率下，观察到了不同的代谢活性：连续培养中葡萄糖和谷氨酰胺的消耗速率比分批培养低40%，但抗体产生速率高70%。在分批培养的初始延迟期也测量到了更高的代谢速率。当将比抗体产生速率的变化表示为比生长速率的函数时，分批培养中生长与抗体产生之间存在正相关，但在连续培养的过渡阶段存在负相关。分批培养和连续培养中细胞代谢的动力学差异可能是细胞生理和代谢发生改变的结果，在连续培养中，细胞广泛暴露于葡萄糖限制条件下。
