Boraston R, Thompson P W, Garland S, Birch J R
Dev Biol Stand. 1983;55:103-11.
Mouse hybridoma cells producing a monoclonal antibody to anti-B blood group antigen have been grown in airlift fermenters up to 30 litres capacity. As a prerequisite for scaling the process up, the oxygen utilization rate of the cells has been established by a dynamic method using an oxygen electrode. The data is compared with that obtained from a chemostat under oxygen limited steady state conditions.
产生抗B血型抗原单克隆抗体的小鼠杂交瘤细胞已在容量达30升的气升式发酵罐中培养。作为扩大该工艺规模的前提条件，已通过使用氧电极的动态方法确定了细胞的氧利用率。将该数据与在氧限制稳态条件下从恒化器获得的数据进行比较。
