Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture.

Mouse hybridoma cells producing a monoclonal antibody to anti-B blood group antigen have been grown in airlift fermenters up to 30 litres capacity. As a prerequisite for scaling the process up, the oxygen utilization rate of the cells has been established by a dynamic method using an oxygen electrode. The data is compared with that obtained from a chemostat under oxygen limited steady state conditions.