Frew T J, Whitaker B A, Greenhouse J J, Hughes S H, Yamamoto H, Takeuchi T, Brumbaugh J A
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
SAAS Bull Biochem Biotechnol. 1990 Jan;3:112-7.
A cDNA encoding mouse tyrosinase was inserted into a plasmid containing the provirus of a replication competent Avian Leukosis Virus (ALV). A viral stock produced from the plasmid was used to infect cultured tyrosinase-negative (ca/ca) unpigmented chick embryo pigment cells. Five days after infection many cells were producing very dark discrete pigment granules. Cultures of tyrosinase positive, sex linked albino (sal) pigment cells produced no additional pigmentation. White Leghorn pigment cells responded to viral infection like the sal pigment cells.
将编码小鼠酪氨酸酶的互补DNA（cDNA）插入到一个含有复制活性禽白血病病毒（ALV）前病毒的质粒中。从该质粒产生的病毒原液用于感染培养的酪氨酸酶阴性（ca/ca）无色素鸡胚色素细胞。感染后五天，许多细胞产生了非常深的离散色素颗粒。酪氨酸酶阳性的性连锁白化病（sal）色素细胞培养物未产生额外色素沉着。白来航色素细胞对病毒感染的反应与sal色素细胞相似。
