Kwon B S, Halaban R, Kim G S, Usack L, Pomerantz S, Haq A K
Molecular Genetics Laboratory, Guthrie Research Institute, Sayre, PA 18840-1692.
Mol Biol Med. 1987 Dec;4(6):339-55.
Two groups of cDNA clones were isolated by screening a lambda gt11 cDNA library of normal human melanocytes with antityrosinase antibodies: one group of 13 was related to the human tyrosinase gene. The properties of the other group of three cDNA clones was investigated by the use of a representative clone, Pmel 17-1. The cDNA hybridized to an mRNA species of approximately 2600 bases from human and murine melanocytes. The transcript of Pmel 17-1 (17-1 mRNA) was expressed preferentially in melanocytes and its abundance paralleled the melanin content. The expression of Pmel 17-1 mRNA increased after stimulation of human and murine melanoma cells with agents that increase the levels of melanization. Immunocompetition assays with monoclonal antibodies to gp75, a known pigmentation-associated antigen of melanocytes, suggested that Pmel 17-1 encodes a 75,000 Mr glycoprotein that is highly abundant in melanotic cells and shares some immunological homology with tyrosinase. The gene for Pmel 17-1 did not map at or near the c-albino locus in mice. The cDNA of Pmel 17-1 detected a single hybridizing restriction fragment in both human and murine DNA, indicating that the gene has been conserved between these two species and exists as a single gene in each.
通过用抗酪氨酸酶抗体筛选正常人黑素细胞的λgt11 cDNA文库，分离出两组cDNA克隆：一组13个与人类酪氨酸酶基因相关。利用一个代表性克隆Pmel 17 - 1对另一组三个cDNA克隆的特性进行了研究。该cDNA与来自人和小鼠黑素细胞的约2600个碱基的mRNA杂交。Pmel 17 - 1的转录本（17 - 1 mRNA）在黑素细胞中优先表达，其丰度与黑色素含量平行。用人和小鼠黑色素瘤细胞用增加黑素化水平的试剂刺激后，Pmel 17 - 1 mRNA的表达增加。用针对gp75（黑素细胞一种已知的色素沉着相关抗原）的单克隆抗体进行免疫竞争分析表明，Pmel 17 - 1编码一种75,000 Mr的糖蛋白，该蛋白在黑素细胞中高度丰富，并且与酪氨酸酶具有一些免疫同源性。Pmel 17 - 1基因不在小鼠的c - 白化病基因座或其附近定位。Pmel 17 - 1的cDNA在人和小鼠DNA中均检测到一个单一的杂交限制性片段，表明该基因在这两个物种之间保守，并且在每个物种中以单基因形式存在。
