Narayanan S R, Crane L J
Research and Development Laboratories, J. T. Baker Inc., Phillipsburg, New Jersey 08865.
Trends Biotechnol. 1990 Jan;8(1):12-6. doi: 10.1016/0167-7799(90)90124-g.
Because of its high selectivity, affinity chromatography is a preferred tool in the downstream processing of high-value proteins and peptides of therapeutic interest. This review examines the affinity supports currently available, and investigates the performance characteristics and properties required of the support matrices for improved affinity-based supports for large-scale purification of biomolecules. Parameters for optimizing an affinity chromatographic process, and the advantages of affinity-based separation for scaled-up systems are highlighted.
由于其高选择性，亲和色谱法是下游处理具有治疗意义的高价值蛋白质和肽的首选工具。本综述研究了目前可用的亲和载体，并探讨了用于改进基于亲和的载体以大规模纯化生物分子的载体基质所需的性能特征和特性。文中强调了优化亲和色谱过程的参数，以及基于亲和的分离对于放大系统的优势。
