Porath J
Institute of Biochemistry, University of Uppsala, Sweden.
J Chromatogr. 1988 Jun 29;443:3-11. doi: 10.1016/s0021-9673(00)94778-2.
The present status of the undeveloped branch of high-performance immobilized-metal-ion affinity chromatography (HPIMAC) is reviewed. As demonstrated, high resolution of peptides and protein mixtures can be obtained. Under specified conditions, more than 1000 runs can be made repeatedly on a single column without much change in pattern or recovery. Occasionally, 10(3)- to 10(4)-fold purification of a protein can be obtained in a single chromatographic run.
本文综述了高性能固定化金属离子亲和色谱法（HPIMAC）尚未充分发展的分支的现状。结果表明，该方法可实现肽和蛋白质混合物的高分辨率分离。在特定条件下，同一根柱子可重复使用1000多次，分离图谱和回收率变化不大。偶尔，在一次色谱运行中就能实现蛋白质10³至10⁴倍的纯化。
