Hussain H A, Ritchie D A
Department of Genetics and Microbiology, Donnan Laboratories, University of Liverpool, UK.
Lett Appl Microbiol. 1990 Nov;11(5):240-3. doi: 10.1111/j.1472-765x.1990.tb00171.x.
Two high copy number, broad host range, general purpose cloning vectors, pLG5 and pLG10, derived from the unstable Streptomyces niveus plasmid pSN2 are described. pLG5 (5.5 kb) and pLG10 (6.5 kb) both carry the thiostrepton resistance (TsrR) and lethal zygosis (Ltz+) markers and have single cloning sites within a non-essential region and the tsr gene. pLG505 (7.4 kb) was constructed by cloning the viomycin resistance (vph) gene into the single BamHI site of pLG5 to give a further vector with insertion and replacement sites which inactivate either the TsrR or VioR functions.
描述了两种高拷贝数、广宿主范围的通用克隆载体pLG5和pLG10，它们衍生自不稳定的雪白链霉菌质粒pSN2。pLG5（5.5 kb）和pLG10（6.5 kb）均携带硫链丝菌素抗性（TsrR）和致死合子形成（Ltz+）标记，并且在非必需区域和tsr基因内具有单克隆位点。通过将紫霉素抗性（vph）基因克隆到pLG5的单个BamHI位点构建了pLG505（7.4 kb），以提供另一种具有插入和替换位点的载体，这些位点可使TsrR或VioR功能失活。
