Bailey C R, Bruton C J, Butler M J, Chater K F, Harris J E, Hopwood D A
J Gen Microbiol. 1986 Aug;132(8):2071-8. doi: 10.1099/00221287-132-8-2071.
The 10.8 kb plasmid pJV1, isolated from Streptomyces phaeochromogenes, has a high copy number (about 150) and a broad host range among Streptomyces spp. Several pJV1 derivatives carrying the thiostrepton resistance gene (tsr) of S. azureus were made. One derivative, pWOR191, was shown to promote its own transfer and to mobilize chromosomal markers in S. lividans. Another derivative, pWOR109, was non-transmissible. Deletion in vitro of a segment of pWOR109 gave pWOR120 (5.6 kb), which has single BamHI and Bg/II sites shown to be capable of accepting 'foreign' DNA such as a previously cloned S. antibioticus DNA fragment encoding tyrosinase, giving vectors (pWOR125, pWOR126) with properties resembling the well-established multicopy vector pIJ702. Shuttle vectors capable of functioning in both S. lividans and Escherichia coli were also constructed. The region of pJV1 essential for replication and maintenance was localized to a 2.5 kb segment. Stable maintenance of pWOR109 and pWOR120 was observed in the presence of derivatives of pIJ101, the progenitor of pIJ702.
从产色链霉菌中分离得到的10.8 kb质粒pJV1，在链霉菌属中具有高拷贝数（约150）和广泛的宿主范围。构建了几种携带天蓝链霉菌硫链丝菌素抗性基因（tsr）的pJV1衍生物。其中一种衍生物pWOR191，已证明能促进自身转移并在变铅青链霉菌中移动染色体标记。另一种衍生物pWOR109则不可转移。在体外对pWOR109的一段进行缺失得到了pWOR120（5.6 kb），它具有单个BamHI和Bg/II位点，已证明能够接受“外源”DNA，例如先前克隆的编码酪氨酸酶的抗生链霉菌DNA片段，从而得到了具有类似于成熟的多拷贝载体pIJ702特性的载体（pWOR125、pWOR126）。还构建了能够在变铅青链霉菌和大肠杆菌中都发挥作用的穿梭载体。pJV1复制和维持所必需的区域定位于一个2.5 kb的片段。在pIJ702的前身pIJ101的衍生物存在的情况下，观察到pWOR109和pWOR120能稳定维持。
