Selenska S, Klingmüller W
Department of Genetics, University of Bayreuth, Germany.
Lett Appl Microbiol. 1991 Jul;13(1):21-4. doi: 10.1111/j.1472-765x.1991.tb00559.x.
Specific Tn5 sequences inserted in the genome of Enterobacter agglomerans were detected in EcoRI digested DNA directly recovered from soil 70 d after its inoculation with the bacteria, when these were no longer culturable on agar medium. A new method of DNA extraction from soil was used. No amplification of DNA sequences by PCR was needed.
