Watarai M, Takeda K, Uesiba H
Department of Public Health, Nihon Veterinary and Animal Science University, Tokyo, Japan.
Nihon Saikingaku Zasshi. 1991 Mar;46(2):529-32. doi: 10.3412/jsb.46.529.
Soil samples taken monthly in 1990 from five parks outside Tokyo were examined for Enterobacter agglomerans. A total of 348 strains were isolated and 250 of them were tested for the presence of plasmids by DNA agarose gel electrophoresis. All the isolates carried at least two kinds of plasmids. Those isolated from July to September showed four or five kinds of plasmids (group I) and those isolated from January to June and from October to December showed two or three kinds of plasmids (group II). A majority of the plasmids detected were of 1,500 or fewer base pairs. The isolates were tested for the Pb2+ resistance; group I strains were more resistant to lead than group II strains. It is presumed that bacterial plasmids are related with the ecosystem of soil and the resistance to lead in E. agglomerans.
1990年每月从东京郊外的五个公园采集土壤样本，检测其中的成团肠杆菌。共分离出348株菌株，其中250株通过DNA琼脂糖凝胶电泳检测质粒的存在。所有分离株至少携带两种质粒。7月至9月分离出的菌株含有四种或五种质粒（第一组），1月至6月以及10月至12月分离出的菌株含有两种或三种质粒（第二组）。检测到的大多数质粒碱基对在1500个及以下。对分离株进行铅离子抗性测试；第一组菌株比第二组菌株对铅的抗性更强。据推测，细菌质粒与土壤生态系统以及成团肠杆菌对铅的抗性有关。
