出芽短梗霉的遗传转化

Genetic transformation of Aureobasidium pullulans.

作者信息

Cullen D, Yang V, Jeffries T, Bolduc J, Andrews J H

机构信息

Institute for Microbial and Biochemical Technology, Forest Products Laboratory, U.S. Department of Agriculture, Madison, Wisconsin 53705-2398.

出版信息

J Biotechnol. 1991 Dec;21(3):283-8. doi: 10.1016/0168-1656(91)90048-z.

DOI:10.1016/0168-1656(91)90048-z

PMID:1369347

Abstract

Aureobasidium pullulans strain Y117 was transformed to hygromycin resistance using plasmid pDH33, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Aspergillus niger glucoamylase gene (glaA). Southern hybridizations of transformants revealed multiple, integrated copies of the vector. The glaA promoter was not induced by starch in A. pullulans as it is in A. niger; however, the transcriptional start points were the same in both species.

摘要

使用质粒pDH33将出芽短梗霉菌株Y117转化为潮霉素抗性，该质粒包含与黑曲霉糖化酶基因（glaA）启动子元件融合的细菌潮霉素B磷酸转移酶基因（hph）。转化体的Southern杂交显示载体有多个整合拷贝。glaA启动子在出芽短梗霉中不像在黑曲霉中那样被淀粉诱导；然而，两个物种的转录起始点是相同的。