Ventura L, Ramón D
Unidad de Bioingeniería de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos (C.S.I.C.), Valencia, Spain.
FEMS Microbiol Lett. 1991 Aug 1;66(2):189-93. doi: 10.1016/0378-1097(91)90331-4.
Aspergillus terreus was transformed to hygromycin B resistance using a bacterial resistance gene under the control of Aspergillus nidulans regulatory sequences. Southern hybridization of transformants indicated that in most of the cases the vector DNA was integrated into the recipient chromosome in the form of tandem arrays. Transformants were mitotically stable in both selective and non-selective medium and retained their capacity to produce xylanase or glucoamylase activities.
利用在构巢曲霉调控序列控制下的细菌抗性基因，将土曲霉转化为对潮霉素B具有抗性。转化体的Southern杂交表明，在大多数情况下，载体DNA以串联阵列的形式整合到受体染色体中。转化体在选择性和非选择性培养基中均具有有丝分裂稳定性，并保留了产生木聚糖酶或葡糖淀粉酶活性的能力。
