Agarwal M, Gaikar V G
Department of Chemical Technology, University of Bombay, Matunga, India.
Bioseparation. 1992;3(4):233-40.
Proteins have been partitioned in poly(ethylene glycol) (PEG)-salt systems containing hydrotropes. Hydrotropes are surface active compounds with strong ionic nature with a smaller hydrophobic part as compared to surfactants. The effect of pH, hydrotrope concentration, polymer molecular weight and protein molecular weight on partitioning of proteins including enzymes and their separation has been investigated. The effects of hydrotropes may be explained on the basis of interaction of PEG and the hydrotropes. This is substantiated by measuring the concentration of hydrotropes in both the phases. The practical utilization of the described effect is to enhance the separation of a mixture of proteins by many folds. Preliminary experiments have shown that hydrotropes at low concentrations do not affect enzyme activity and do not have any bactericidal activity.
蛋白质已在含有助水溶物的聚乙二醇（PEG）-盐体系中进行了分配。助水溶物是具有强离子性质的表面活性化合物，与表面活性剂相比，其疏水部分较小。研究了pH值、助水溶物浓度、聚合物分子量和蛋白质分子量对包括酶在内的蛋白质分配及其分离的影响。助水溶物的作用可以基于PEG与助水溶物的相互作用来解释。通过测量两相中助水溶物的浓度证实了这一点。所述作用的实际应用是将蛋白质混合物的分离提高许多倍。初步实验表明，低浓度的助水溶物不会影响酶活性，也没有任何杀菌活性。
