Lu M, Johansson G, Albertsson P A, Tjerneld F
Department of Biochemistry, University of Lund, Sweden.
Bioseparation. 1995;5(6):351-8.
Partitioning of proteins was studied in aqueous two-phase systems composed of the polymers dextran and hydrophobically modified dextran. The modified dextrans were benzoyl dextran with a degree of substitution of 0.17 and valeryl dextran with a degree of substitution of 0.20. Phase diagrams for the systems of dextran/benzoyl dextran and dextran/valeryl dextran were determined at room temperature. The proteins studied were beta-galactosidase, bovine serum albumin, beta-lactoglobulin, lysozyme, myoglobin and cytochrome C. The partition coefficients of a series of salts were determined in dextran/benzoyl dextran two-phase systems. The addition of salts had strong effect on the partitioning of proteins. This effect was related to protein net charge and the position of the ions in the Hofmeister series. Cross partitioning of bovine serum albumin was studied in a dextran/benzoyl dextran aqueous two-phase system.
在由聚合物葡聚糖和疏水改性葡聚糖组成的双水相系统中研究了蛋白质的分配。改性葡聚糖为取代度为0.17的苯甲酰葡聚糖和取代度为0.20的戊酰葡聚糖。在室温下测定了葡聚糖/苯甲酰葡聚糖和葡聚糖/戊酰葡聚糖体系的相图。所研究的蛋白质有β-半乳糖苷酶、牛血清白蛋白、β-乳球蛋白、溶菌酶、肌红蛋白和细胞色素C。在葡聚糖/苯甲酰葡聚糖双水相系统中测定了一系列盐的分配系数。盐的加入对蛋白质的分配有强烈影响。这种影响与蛋白质净电荷和离子在霍夫迈斯特序列中的位置有关。在葡聚糖/苯甲酰葡聚糖双水相系统中研究了牛血清白蛋白的交叉分配。
