在LE和Wistar/Shhi大鼠中诱导的升结肠癌以及携带WF大鼠结肠癌血清中的病毒样颗粒。
Induced adenocarcinoma of the ascending colon on LE and Wistar/Shhi rats and virus like particles in the serum of colon cancer carrying WF rats.
作者信息
Miyamoto M, Saeki K, Tani Y, Amano H, Sodoh T O, Nakao H, Konishi T
机构信息
Department of Pathology, Osaka University Medical School, Japan.
出版信息
Med J Osaka Univ. 1991 Mar;40(1-4):5-7.
Intraperitoneal injection of the serum of colon cancer carrying WF rats induced, within two months, colon carcinomas in the ascending colon of LE and Wistar/Shi rats when they were given it during their suckling. We had also induced colon carcinomas in the ascending colon of ACI rats by the same methods. Therefore, it is supported that this serum derived from colon cancer carrying WF rats must have some transmissible agent in itself. In addition, we ultracentrifuged the serum of cancer carrying WF rats and we found, in the sediment, numerous round or oval virus like corpuscles by electron microscopy studies. Negatively stained corpuscles by phosphotungstic acid staining clearly revealed fine spike appearance on their surface. We believe that these virus like corpuscles are the etiological agent for the transmissible colon carcinoma of WF rat strain.
给正在哺乳的LE和Wistar/Shi大鼠腹腔注射携带结肠癌的WF大鼠的血清，两个月内可在其升结肠诱发结肠癌。我们还用同样的方法在ACI大鼠的升结肠诱发了结肠癌。因此，有证据支持，这种来自携带结肠癌的WF大鼠的血清本身必定含有某种可传播因子。此外，我们对携带癌症的WF大鼠的血清进行了超速离心，通过电子显微镜研究在沉淀物中发现了许多圆形或椭圆形的类病毒小体。经磷钨酸染色的负染小体在其表面清晰地显示出细小的刺突外观。我们认为这些类病毒小体是WF大鼠品系可传播结肠癌的病原体。
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