Shrager R I, Mihalyi E, Towne D W
Biochemistry. 1976 Nov 30;15(24):7382-6. doi: 10.1021/bi00669a026.
The kinetic data presented in the previous paper (Mihalyi, E., et al. (1976), Biochemistry 15, preceding paper in this issue), with respect to the fragmentation of human the bovine fibrinogen by either plasmin or trypsin, were compared with several chemical kinetic models. The models were derived mathematically on the basis of the three-nodular structure of fibrinogen (Hall, C.E., and Sayter, H.S. (1959), J. BiophysBiochem. Ctyol. 5, 11) and the asymmetrical cleavage sequence first proposed by Marder, V.J., et. al. ((1969) J. Biol. Chem. 244, 2111). The parameters were determined by nonlinear curve fitting. The whole process could be described accurately by only two rate constants. Several variant models were tested and, although a clear cut choice cannot be made, one of these, the protected three-bonds model, appears to give the best fit in most cases. This model assumes that the chain segment that distinguishes F from X protects certain other chains (the bonds) from proteolytic cleavage.
将前一篇论文（米哈伊，E.等人（1976年），《生物化学》第15卷，本期前一篇论文）中关于纤溶酶或胰蛋白酶对人及牛纤维蛋白原片段化的动力学数据，与几种化学动力学模型进行了比较。这些模型是根据纤维蛋白原的三结节结构（霍尔，C.E.和赛特，H.S.（1959年），《生物物理与生物化学细胞杂志》第5卷，第11页）以及马德，V.J.等人首先提出的不对称裂解序列（（1969年）《生物化学杂志》第244卷，第2111页）通过数学推导得出的。参数通过非线性曲线拟合确定。整个过程仅用两个速率常数就能准确描述。测试了几种变体模型，尽管无法做出明确选择，但其中一种，即受保护的三键模型，在大多数情况下似乎拟合得最好。该模型假设区分F和X的链段会保护其他某些链（键）不被蛋白水解裂解。
