人纤维蛋白原D片段的结晶
Crystallization of fragment D from human fibrinogen.
作者信息
Everse S J, Pelletier H, Doolittle R F
机构信息
Department of Chemistry, University of California at San Diego, La Jolla 92093-0634, USA.
出版信息
Protein Sci. 1995 May;4(5):1013-6. doi: 10.1002/pro.5560040523.
Abstract
Fragment D from human fibrinogen has been crystallized. The fragment, which is composed of three disulfide-linked chains (alpha' beta' gamma' = 88,000), was generated with either plasmin or mild trypsin digestion. The crystals diffracted out to 3.5 A; the space group is P2(1), unit cell dimensions a = 108 A, b = 48 A, c = 167 A, beta = 106 degrees. Fragment D was also co-crystallized with the ligand GPRP-amide, in which case the space group is consistent with P212121, unit cell dimensions a = 476 A, b = 82 A, c = 432 A.
摘要
人纤维蛋白原的D片段已结晶。该片段由三条通过二硫键连接的链（α'β'γ' = 88,000）组成，通过纤溶酶或温和的胰蛋白酶消化产生。晶体衍射分辨率达到3.5 Å；空间群为P2(1)，晶胞参数a = 108 Å，b = 48 Å，c = 167 Å，β = 106°。D片段还与配体GPRP-酰胺共结晶，此时空间群与P212121一致，晶胞参数a = 476 Å，b = 82 Å，c = 432 Å。
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