肉毒梭菌毒素免疫研究。VII. 胰蛋白酶激活的E型毒素的纯化与解毒
Studies on immunity to toxins of Clostridium botulinum. VII. Purification and detoxification of trypsin-activated type E toxin.
作者信息
FIOCK M A, YARINSKY A, DUFF J T
出版信息
J Bacteriol. 1961 Jul;82(1):66-71. doi: 10.1128/jb.82.1.66-71.1961.
Fiock, Mary A. (Fort Detrick, Frederick, Md.), Allen Yarinsky, and James T. Duff. Studies on immunity to toxins of Clostridium botulinum. VII. Purification and detoxification of trypsin-activated type E toxin. J. Bacteriol. 82:66-71. 1961.-A procedure was described for production, purification, and conversion to toxoid of the Clostridium botulinum type E trypsin-treated toxin. A medium containing 1.5% trypticase, 0.5% yeast extract, 0.075% cysteine hydrochloride, and 1.0% glucose consistently yielded activated culture toxicities of 300,000 mouse intraperitoneal ld(50)/ml. Purification of the toxin was accomplished by precipitation with ammonium sulfate, extraction with calcium chloride, and reprecipitation with ethanol in the cold. The resulting partially purified toxin had a specific activity of 45,000,000 ld(50)/mg nitrogen. Purified toxins were converted to toxoid by incubation with formalin and adsorbed on aluminum phosphate. Good immune responses were obtained to the toxoids in mice, guinea pigs, and rabbits.
菲奥克，玛丽·A.（马里兰州弗雷德里克市迪特里克堡），艾伦·亚林斯基，以及詹姆斯·T.达夫。肉毒梭菌毒素免疫研究。VII. 胰蛋白酶激活的E型毒素的纯化与解毒。《细菌学杂志》82:66 - 71。1961年。——描述了一种生产、纯化肉毒梭菌E型经胰蛋白酶处理的毒素并将其转化为类毒素的方法。一种含有1.5%胰蛋白胨、0.5%酵母提取物、0.075%盐酸半胱氨酸和1.0%葡萄糖的培养基始终能产生300,000小鼠腹腔注射半数致死量（ld(50)）/毫升的活化培养物毒性。毒素的纯化通过硫酸铵沉淀、氯化钙提取以及在低温下用乙醇再沉淀来完成。所得部分纯化的毒素比活性为45,000,000 ld(50)/毫克氮。纯化的毒素通过与福尔马林孵育转化为类毒素，并吸附在磷酸铝上。在小鼠、豚鼠和兔子中对类毒素获得了良好的免疫反应。
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