E型肉毒梭菌毒素的纯化及分子量测定

PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN.

作者信息

GERWING J, DOLMAN C E, REICHMANN M E, BAINS H S

出版信息

J Bacteriol. 1964 Jul;88(1):216-9. doi: 10.1128/jb.88.1.216-219.1964.

DOI:10.1128/jb.88.1.216-219.1964

PMID:14197891

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC277279/

Abstract

Gerwing, Julia (The University of British Columbia, Vancouver, British Columbia, Canada), Claude E. Dolman, M. E. Reichmann, and Hardial S. Bains. Purification and molecular weight determination of Clostridium botulinum type E toxin. J. Bacteriol. 88:216-219. 1964.-A method was developed whereby type E botulinus toxin can be obtained in a highly purified state by elution through acidified diethylaminoethyl-cellulose columns. The material thus isolated appears to be electrophoretically and ultracentrifugally homogeneous. A molecular weight of 18,600 was calculated for the toxin.

摘要

格温，朱莉娅（加拿大不列颠哥伦比亚省温哥华市英属哥伦比亚大学），克劳德·E·多尔曼、M·E·赖克曼和哈迪阿尔·S·贝恩斯。肉毒梭菌E型毒素的纯化及分子量测定。《细菌学杂志》88:216 - 219。1964年。——已开发出一种方法，通过酸化二乙氨基乙基纤维素柱洗脱，可获得高度纯化的E型肉毒杆菌毒素。如此分离得到的物质在电泳和超速离心方面似乎是均一的。计算出该毒素的分子量为18,600。