脱氧核糖核酸产物对有毒和无毒肺炎球菌脱氧核糖核酸合成的影响。
Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci.
作者信息
FIRSHEIN W
出版信息
J Bacteriol. 1961 Aug;82(2):169-80. doi: 10.1128/jb.82.2.169-180.1961.
Abstract
Firshein, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J. Bacteriol. 82:169-180. 1961.-Cell suspensions of virulent pneumococci synthesize a greater amount of deoxyribonucleic acid (DNA) per cell in the presence of DNA + deoxyribonuclease + mixtures of deoxynucleosides and deoxynucleotides (supplement-1) than unsupplemented suspensions. Under identical conditions, avirulent and weakly virulent pneumococci do not respond to these DNA breakdown products. Glucose and casitone must be present for maximal effects to occur.A DNA turnover occurs in virulent cells. This has been demonstrated by a decrease in specific activity of H(3)-DNA extracted from virulent (S) cells exposed to nonradioactive supplement-1.
摘要
菲尔申，W.（卫斯理大学，康涅狄格州米德尔敦）。脱氧核糖核酸产物对有毒和无毒肺炎球菌脱氧核糖核酸合成的影响。《细菌学杂志》82:169 - 180。1961年。——在存在DNA + 脱氧核糖核酸酶 + 脱氧核苷和脱氧核苷酸混合物（补充物 - 1）的情况下，有毒肺炎球菌的细胞悬液比未添加补充物的悬液每个细胞合成更多的脱氧核糖核酸（DNA）。在相同条件下，无毒和弱毒肺炎球菌对这些DNA分解产物没有反应。必须存在葡萄糖和酪蛋白胨才能产生最大效果。有毒细胞中发生DNA周转。这已通过从暴露于非放射性补充物 - 1的有毒（S）细胞中提取的H(3)-DNA比活性降低得到证明。
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