N-月桂酰生物素酰胺作为亲和表面活性剂。

N-laurylbiotinamide as affinity surfactant.

作者信息

Coughlin R W, Baclaski J B

机构信息

Department of Chemical Engineering, University of Connecticut, Storrs 06269.

出版信息

Biotechnol Prog. 1990 Jul-Aug;6(4):307-9. doi: 10.1021/bp00004a012.

DOI:10.1021/bp00004a012

PMID:1370004

Abstract

N-laurylbiotinamide (NLB), which retains strong affinity for the protein avidin, was synthesized from biotin and N-laurylamine via the biotin ester of N-hydroxysuccinimide and characterized by NMR. When the synthesized NLB was used as a cosurfactant with AOT to form a reverse micellar system in isooctane, it was found to extend the pH range over which avidin can be transferred from a continuous aqueous solution to the reverse-micellar phase. This behavior is similar to that already reported for a different affinity surfactant, n-octyl beta-D-glucopyranoside.

摘要

N-月桂酰生物素酰胺（NLB）对抗生物素蛋白具有很强的亲和力，它由生物素和N-月桂胺通过N-羟基琥珀酰亚胺的生物素酯合成，并通过核磁共振进行了表征。当将合成的NLB与AOT作为共表面活性剂在异辛烷中形成反胶束体系时，发现它能扩展抗生物素蛋白从连续水溶液转移到反胶束相的pH范围。这种行为与已报道的另一种具有不同亲和力的表面活性剂正辛基-β-D-吡喃葡萄糖苷的行为相似。