Hartounian H, Sandler S I
Department of Chemical Engineering, University of Delaware, Newark 19716.
Biotechnol Prog. 1991 May-Jun;7(3):279-82. doi: 10.1021/bp00009a012.
We consider the effects of the addition of poly(ethylene glycol) (PEG) of different molecular weights to aqueous two-phase system of PEG 8000 and dextran 500. The first purpose of this study was to determine the molecular weight partitioning of the polymers themselves so that, for example, aqueous two-phase separations using affinity ligands can be improved. The second purpose was to examine whether this molecular weight partitioning could be predicted by using solution thermodynamic models so that it would be possible to optimize affinity partitioning without extensive laboratory work. Experimentally, we find that, by increasing the PEG concentration of any molecular weight in the feed, the high molecular weight PEG concentration in the dextran-rich phase is reduced. This observation can be used to reduce the loss of expensive ligated PEG used in affinity partitioning. Further, there is generally good agreement between our experimental data and the predictions of a solution thermodynamic model.
我们研究了向聚乙二醇8000和葡聚糖500的双水相体系中添加不同分子量的聚乙二醇（PEG）所产生的影响。本研究的首要目的是确定聚合物本身的分子量分配情况，以便例如能够改进使用亲和配体的双水相分离。第二个目的是检验这种分子量分配是否可以通过溶液热力学模型进行预测，从而有可能在无需大量实验室工作的情况下优化亲和分配。通过实验，我们发现，通过增加进料中任何分子量的PEG浓度，富含葡聚糖相中的高分子量PEG浓度会降低。这一观察结果可用于减少亲和分配中使用的昂贵连接PEG的损失。此外，我们的实验数据与溶液热力学模型的预测结果总体上吻合良好。
