化脓性链球菌（A 组）M12 型 A374 菌株链激酶的免疫化学研究及完整氨基酸序列

Ohkuni H, Todome Y, Suzuki H, Mizuse M, Kotani N, Horiuchi K, Shikama N, Tsugita A, Johnston K H

Division of Immunology, Nippon Medical School, Kanagawa, Japan.

Infect Immun. 1992 Jan;60(1):278-83. doi: 10.1128/iai.60.1.278-283.1992.

The complete amino acid sequence of the streptokinase (SKase) of Streptococcus pyogenes M type 12 strain A374, isolated from a patient with poststreptococcal glomerulonephritis (PSGN), was determined. The epitope domain for the monoclonal antibody N-59, which cross-reacts with SKases of both the PSGN-associated strain and S. equisimilis H46A (a non-PSGN-associated strain), was predicted to be localized in residues 370 to 374. The epitope domain specific for monoclonal antibody RU-1, which reacts only with the PSGN-associated SKase, was localized to residues 164 to 236.

已确定从一名患有链球菌感染后肾小球肾炎（PSGN）的患者分离出的化脓性链球菌M12型A374菌株的链激酶（SKase）的完整氨基酸序列。预测与PSGN相关菌株和马链球菌兽疫亚种H46A（非PSGN相关菌株）的SKases均发生交叉反应的单克隆抗体N-59的表位域位于第370至374位残基。仅与PSGN相关的SKase发生反应的单克隆抗体RU-1的特异性表位域定位于第164至236位残基。