用单克隆抗体检测肾炎菌株相关链激酶。

Detection of nephritis strain-associated streptokinase by monoclonal antibodies.

作者信息

Ohkuni H, Todome Y, Yoshimura K, Yamamoto T, Suzuki H, Yokomuro K, Johnston K H, Zabriskie J B

机构信息

Division of Immunology, Nippon Medical School, Kanagawa, Japan.

出版信息

J Med Microbiol. 1991 Jul;35(1):60-3. doi: 10.1099/00222615-35-1-60.

DOI:10.1099/00222615-35-1-60

PMID:2072380

Abstract

Monoclonal antibodies (MAbs) N-59 and RU-1 were produced by immunisation of mice with streptokinase secreted by Streptococcus group A, type 12, strain A374 isolated from a patient with post-streptococcal glomerulonephritis (PSGN) and were characterised by Western blot analysis. MAb N-59 recognised antigenic determinants shared by both nephritis strain-associated streptokinase (NSA-SKase) and streptokinase of Streptococcus group C (C-SKase); MAb RU-1 reacted only with NSA-SKase. All nephritis-associated group A streptococcal strains tested reacted with MAb N-59; 87.5% of these strains reacted with MAb RU-1. MAb N-59 reacted with SKase produced by group G streptococcal strains isolated from patients with PSGN, and MAb RU-1 recognised SKase in two out of three of these strains.

摘要

用从一名链球菌感染后肾小球肾炎（PSGN）患者分离出的A组12型A374菌株分泌的链激酶免疫小鼠，制备了单克隆抗体（MAb）N - 59和RU - 1，并通过蛋白质印迹分析对其进行了表征。单克隆抗体N - 59识别肾炎菌株相关链激酶（NSA - SKase）和C组链球菌链激酶（C - SKase）共有的抗原决定簇；单克隆抗体RU - 1仅与NSA - SKase反应。所有测试的与肾炎相关的A组链球菌菌株均与单克隆抗体N - 59反应；其中87.5%的菌株与单克隆抗体RU - 1反应。单克隆抗体N - 59与从PSGN患者分离出的G组链球菌菌株产生的SKase反应，单克隆抗体RU - 1在其中三分之二的菌株中识别出SKase。