Maslov D A, Sturm N R, Niner B M, Gruszynski E S, Peris M, Simpson L
Department of Biology, University of California, Los Angeles 90024.
Mol Cell Biol. 1992 Jan;12(1):56-67. doi: 10.1128/mcb.12.1.56-67.1992.
Six short G-rich intergenic regions in the maxicircle of Leishmania tarentolae are conserved in location and polarity in two other kinetoplastid species. We show here that G-rich region 6 (G6) represents a pan-edited cryptogene which contains at least two domains edited independently in a 3'-to-5' manner connected by short unedited regions. In the completely edited RNA, 117 uridines are added at 49 sites and 32 uridines are deleted at 13 sites, creating a translated 85-amino-acid polypeptide. Similar polypeptides are probably encoded by pan-edited G6 transcripts in two other species. The G6 polypeptide has significant sequence similarity to the family of S12 ribosomal proteins. A minicircle-encoded gRNA overlaps 12 editing sites in G6 mRNA, and chimeric gRNA/mRNA molecules were shown to exist, in agreement with the transesterification model for editing.
大利什曼原虫（Leishmania tarentolae）大环中的六个富含鸟嘌呤的短基因间区域在位置和极性上在另外两种动质体物种中是保守的。我们在此表明，富含鸟嘌呤的区域6（G6）代表一个泛编辑隐基因，它包含至少两个以3'到5'方式独立编辑的结构域，由短的未编辑区域连接。在完全编辑的RNA中，在49个位点添加了117个尿苷，在13个位点删除了32个尿苷，产生了一个翻译后的85个氨基酸的多肽。另外两个物种中可能由泛编辑的G6转录本编码类似的多肽。G6多肽与S12核糖体蛋白家族具有显著的序列相似性。一个小环编码的引导RNA（gRNA）与G6 mRNA中的12个编辑位点重叠，并且已证明存在嵌合的gRNA/mRNA分子，这与编辑的转酯模型一致。
