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动质体纲黑海分离株的线粒体基因组序列

Mitogenome sequence of a Black Sea isolate of the kinetoplastid .

作者信息

Gastineau Romain, Lemieux Claude, Turmel Monique, Davidovich Nikolaï A, Davidovich Olga I, Mouget Jean-Luc, Witkowski Andrzej

机构信息

Natural Sciences Research and Educational Center and Palaeoceanology Unit, Faculty of Geosciences, University of Szczecin, Szczecin, Poland.

Département de biochimie, de microbiologie et de bio-informatique, Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, Canada.

出版信息

Mitochondrial DNA B Resour. 2018 Sep 10;3(2):968-969. doi: 10.1080/23802359.2018.1507654.

DOI:10.1080/23802359.2018.1507654
PMID:33474382
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7799735/
Abstract

We obtained the mitogenome sequence of a Black Sea isolate of the kinetoplastid . This sequence consists of two contigs totaling 24,925 bp and encodes ten protein-coding genes, one conserved ORF and one rRNA gene. Alignment of the Black Sea mitogenome with the limited sequence data currently available in public databases for another strain of revealed significant genetic divergence between the two isolates. Maximum likelihood phylogenetic inference clearly resolved the Bodonidae from the Trypanosomatidae.

摘要

我们获得了一种动质体黑海分离株的线粒体基因组序列。该序列由两个重叠群组成,总长24,925 bp,编码十个蛋白质编码基因、一个保守开放阅读框和一个核糖体RNA基因。将黑海线粒体基因组与公共数据库中目前可获得的另一种菌株的有限序列数据进行比对,结果显示这两种分离株之间存在显著的遗传差异。最大似然系统发育推断清楚地将波豆科与锥虫科区分开来。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cad9/7799735/94b40f1bfe42/TMDN_A_1507654_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cad9/7799735/94b40f1bfe42/TMDN_A_1507654_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cad9/7799735/94b40f1bfe42/TMDN_A_1507654_F0001_B.jpg

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本文引用的文献

1
Comparative Metabolism of Free-living Bodo saltans and Parasitic Trypanosomatids.自由生活的波豆虫与寄生性锥虫的比较代谢
J Eukaryot Microbiol. 2016 Sep;63(5):657-78. doi: 10.1111/jeu.12315. Epub 2016 Apr 20.
2
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.MEGA6:分子进化遗传学分析版本 6.0。
Mol Biol Evol. 2013 Dec;30(12):2725-9. doi: 10.1093/molbev/mst197. Epub 2013 Oct 16.
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SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing.SPAdes:一种新的基因组组装算法及其在单细胞测序中的应用
J Comput Biol. 2012 May;19(5):455-77. doi: 10.1089/cmb.2012.0021. Epub 2012 Apr 16.
4
Insights into the genome sequence of a free-living Kinetoplastid: Bodo saltans (Kinetoplastida: Euglenozoa).对自由生活的动基体生物——波豆虫(动基体目:眼虫纲)基因组序列的见解。
BMC Genomics. 2008 Dec 9;9:594. doi: 10.1186/1471-2164-9-594.
5
Molecular comparisons of freshwater and marine isolates of the same morphospecies of heterotrophic flagellates.淡水与海洋中异养鞭毛虫相同形态种分离株的分子比较。
Appl Environ Microbiol. 2006 Oct;72(10):6638-43. doi: 10.1128/AEM.02547-05.
6
Trypanosoma cruzi mitochondrial maxicircles display species- and strain-specific variation and a conserved element in the non-coding region.克氏锥虫线粒体大环显示出物种和菌株特异性变异以及非编码区的一个保守元件。
BMC Genomics. 2006 Mar 22;7:60. doi: 10.1186/1471-2164-7-60.
7
Kinetoplast DNA network: evolution of an improbable structure.动质体DNA网络:一种奇特结构的演化
Eukaryot Cell. 2002 Aug;1(4):495-502. doi: 10.1128/EC.1.4.495-502.2002.
8
Phylogeny of the bodonid flagellates (Kinetoplastida) based on small-subunit rRNA gene sequences.基于小亚基核糖体RNA基因序列的波豆鞭毛虫(动质体目)系统发育研究
Int J Syst Evol Microbiol. 2000 Sep;50 Pt 5:1943-1951. doi: 10.1099/00207713-50-5-1943.
9
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Nucleic Acids Res. 1998 Mar 1;26(5):1205-13. doi: 10.1093/nar/26.5.1205.
10
Primary sequence and partial secondary structure of the 12S kinetoplast (mitochondrial) ribosomal RNA from Leishmania tarentolae: conservation of peptidyl-transferase structural elements.来自热带利什曼原虫的12S动质体(线粒体)核糖体RNA的一级序列和部分二级结构:肽基转移酶结构元件的保守性
Nucleic Acids Res. 1985 Apr 11;13(7):2337-56. doi: 10.1093/nar/13.7.2337.