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[对大鼠主动脉细胞传代培养时所形成的细胞外大分子网络的研究]

[Study of the extracellular macromolecular meshwork elaborated by rat aortic cells in secondary culture].

作者信息

Desgranges C, Razaka G, Larrue J, Bricaud H

出版信息

C R Seances Soc Biol Fil. 1976;170(4):760-4.

PMID:137038
Abstract

Rat aortic smooth muscle cells isolated by digestion of the vessels by elastase and trypsin and grown in subculture, are examinated by phase, optic and electron microscopy for their ability to synthesize connective tissue components. Large amounts of extracellular material accumulates within the spaces between the cell; it consists of amorphous substance identified histochemically as elastin, of 110 A microfibrils and of periodic fibrils (430-490 A); the chemical nature of these two last components is discussed.

摘要

通过用弹性蛋白酶和胰蛋白酶消化血管分离并传代培养的大鼠主动脉平滑肌细胞,用相差显微镜、光学显微镜和电子显微镜检查其合成结缔组织成分的能力。大量细胞外物质积聚在细胞间的间隙中;它由组织化学鉴定为弹性蛋白的无定形物质、110埃的微原纤维和周期性纤维(430 - 490埃)组成;讨论了这后两种成分的化学性质。

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