Baba T, Takagi M, Yagasaki H
Anat Rec. 1985 Nov;213(3):385-91. doi: 10.1002/ar.1092130304.
Two distinct ultrastructural components of elastic fibers can be identified--namely, the amorphous elastin and the microfibrils. We have examined the tunica adventitia of monkey aortas to demonstrate differential localization of carbohydrates in elastic fibers and collagen fibrils using Thiéry's periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining of thin sections for vicinal-glycol-containing complex carbohydrates, en bloc concanavalin A (Con A) staining specific for alpha-D-mannosyl and alpha-D-glucosyl groups, and en bloc wheat germ agglutinin (WGA) staining specific for N-acetyl-D-glucosamine, N-acetylneuraminic acid, and N-acetyl-D-galactosamine. The PA-TCH-SP method moderately stained microfibrils and weakly stained collagen fibrils, but did not stain the amorphous elastin. Both Con A and WGA staining methods strongly stained microfibrils and moderately stained collagen fibrils, whereas the amorphous elastin lacked staining. Thus PA-TCH-SP, Con A, and WGA staining methods allow differential ultrastructural localization of carbohydrates in elastic fibers and collagen fibrils in monkey aortic adventitia and demonstrate the presence of more carbohydrate components in microfibrils than in collagen fibrils, whereas amorphous elastin lacks carbohydrate staining.
弹性纤维有两种不同的超微结构成分，即无定形弹性蛋白和微原纤维。我们检查了猴主动脉的外膜，使用蒂埃里的高碘酸-硫代碳酰肼-银蛋白染色法（PA-TCH-SP）对薄切片进行染色，以显示弹性纤维和胶原纤维中碳水化合物的差异定位，该方法用于检测含邻位二醇的复合碳水化合物；使用伴刀豆球蛋白A（Con A）整体染色法，该方法对α-D-甘露糖基和α-D-葡萄糖基具有特异性；使用麦胚凝集素（WGA）整体染色法，该方法对N-乙酰-D-葡萄糖胺、N-乙酰神经氨酸和N-乙酰-D-半乳糖胺具有特异性。PA-TCH-SP方法对微原纤维染色适中，对胶原纤维染色较弱，但对无定形弹性蛋白不染色。Con A和WGA染色方法对微原纤维染色强烈，对胶原纤维染色适中，而无定形弹性蛋白无染色。因此，PA-TCH-SP、Con A和WGA染色方法可对猴主动脉外膜弹性纤维和胶原纤维中的碳水化合物进行超微结构差异定位，并证明微原纤维中碳水化合物成分比胶原纤维中更多，而无定形弹性蛋白无碳水化合物染色。
