Isokawa K, Takagi M, Toda Y
Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan.
Anat Rec. 1989 Feb;223(2):158-64. doi: 10.1002/ar.1092230207.
In the ventral aorta of lamprey, microfibrils are major components of the extracellular matrix. With special reference to these microfibrils, we have cytochemically examined the lamprey ventral aorta, utilizing the tannic acid (pH 7.0)-uranyl acetate (TA-UA) method, elastase en bloc digestion, Thiéry's periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) method, and ferritin- or horseradish peroxidase-labeled concanavalin A (Con A) methods. The lamprey microfibrils were strongly stained with PA-TCH-SP and both Con A methods, but did not show TA-UA staining nor elastase sensitivity. These cytochemical properties of lamprey microfibrils are identical with those of mammalian elastin-associated microfibrils. On the other hand, in spite of extensive examination, TA-UA positive and elastase-sensitive extracellular components were not found, so that lamprey ventral aorta does not appear to contain elastin. These results indicate that lamprey aortic connective tissue contains microfibrils as elastic components, but deposition of amorphous elastin does not occur.
在七鳃鳗的腹主动脉中，微原纤维是细胞外基质的主要成分。针对这些微原纤维，我们利用单宁酸（pH 7.0）-醋酸铀（TA-UA）法、弹性蛋白酶整体消化法、蒂埃里高碘酸-硫代碳酰肼-银蛋白法（PA-TCH-SP）以及铁蛋白或辣根过氧化物酶标记的伴刀豆球蛋白A（Con A）法，对七鳃鳗腹主动脉进行了细胞化学检查。七鳃鳗的微原纤维被PA-TCH-SP以及两种Con A法强烈染色，但未显示TA-UA染色，也不具有弹性蛋白酶敏感性。七鳃鳗微原纤维的这些细胞化学特性与哺乳动物弹性蛋白相关微原纤维的特性相同。另一方面，尽管进行了广泛检查，但未发现TA-UA阳性且对弹性蛋白酶敏感的细胞外成分，因此七鳃鳗腹主动脉似乎不含有弹性蛋白。这些结果表明，七鳃鳗主动脉结缔组织含有作为弹性成分的微原纤维，但无定形弹性蛋白的沉积并未发生。
