Benedetto A, Di Caro A, Camporiondo M P, Gallone D, Zaniratti S, Tozzi V, Elia G
Center of Virology USL, Osp. S. Camillo, Rome, Italy.
Clin Immunol Immunopathol. 1992 Feb;62(2):139-47. doi: 10.1016/0090-1229(92)90066-w.
We have studied 61 HIV-seropositive heroin addicts (18 asymptomatic, 20 ARC, and 23 AIDS cases), 26 HIV-seronegative heroin addicts, and 45 healthy blood donors, matching the groups each other for age and sex. We have focused on the phenotypic characteristics of B subpopulations in the peripheral blood of HIV-seropositive and -seronegative drug abusers, paying particular attention to the consistence of the "CD20+" B cell subset, which poorly expresses the CD21 membrane receptor for the C3d and Epstein-Barr virus (EBV) (referred to as "CD20 + CD21-" subset). In healthy blood donors, the ratio CD20 + CD21-/CD20+ x 100 is extremely low (mean +/- SEM = 8.1 +/- 0.9) and rarely exceeds the value of 20. On the contrary, in HIV seropositives, the values are much more dispersed, with higher mean values (mean +/- SEM = 25.8 +/- 1.8) ranging from 50 to 60. An intermediate situation characterizes the class of HIV-seronegative heroin addicts, whose values are slightly higher and more dispersed than that of normal controls (mean +/- SEM = 11.6 +/- 1.3). The extent of the amplification of the CD20 + CD21- subset in HIV-seropositive individuals does not apparently correlate with the progression of the disease and represents an early event in the clinical course of HIV infection. For each subject of the study group, the number of CD20 + CD21- B lymphocytes is not correlated to other early markers of HIV infection, as the T4 lymphocyte number, or total Ig levels in sera. A functional characterization of the CD20 + CD21- B cell subset indicates that, in HIV-seropositive patients, these cells are unable to produce specific and nonspecific immunoglobulins (Ig's), either spontaneously or after pokeweed mitogen stimulation. Furthermore, this cell subset is characterized by poor expression of surface Ig's. The data reported suggest that this cell subset can be regarded as situated at an early level of B cell lineage differentiation.
我们研究了61名HIV血清阳性的海洛因成瘾者（18名无症状者、20名艾滋病相关综合征患者和23名艾滋病患者）、26名HIV血清阴性的海洛因成瘾者以及45名健康献血者，并使这些组在年龄和性别上相互匹配。我们重点关注了HIV血清阳性和血清阴性药物滥用者外周血中B亚群的表型特征，尤其关注“CD20+”B细胞亚群的一致性，该亚群低表达C3d和EB病毒（EBV）的CD21膜受体（称为“CD20+CD21-”亚群）。在健康献血者中，CD20+CD21-/CD20+×100的比值极低（平均值±标准误=8.1±0.9），很少超过20。相反，在HIV血清阳性者中，该值分布更为分散，平均值较高（平均值±标准误=25.8±1.8），范围在50至60之间。HIV血清阴性的海洛因成瘾者则处于中间状态，其值比正常对照组略高且更分散（平均值±标准误=11.6±1.3）。HIV血清阳性个体中CD20+CD21-亚群的扩增程度显然与疾病进展无关，是HIV感染临床过程中的一个早期事件。对于研究组的每个受试者，CD20+CD21-B淋巴细胞的数量与HIV感染的其他早期标志物，如T4淋巴细胞数量或血清总Ig水平均无相关性。对CD20+CD21-B细胞亚群的功能特性分析表明，在HIV血清阳性患者中，这些细胞无论是自发还是在商陆有丝分裂原刺激后，均无法产生特异性和非特异性免疫球蛋白（Ig）。此外，该细胞亚群的特点是表面Ig表达不佳。所报道的数据表明，该细胞亚群可被视为处于B细胞谱系分化的早期阶段。
