Monnier D, Colas J F, Rosay P, Hen R, Borrelli E, Maroteaux L
Laboratoire de Génétique des Eucaryotes, Centre National de la Recherche Scientifique, l'Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine de Strasbourg, France.
J Biol Chem. 1992 Jan 15;267(2):1298-302.
A number of neuropeptides have been described which are present in the insect nervous system. The physiological role of these neuropeptides has not yet been clarified. We have characterized a Drosophila melanogaster cDNA coding for a protein, NKD, whose sequence resembles that of mammalian G protein-coupled neuropeptide receptors. This protein shows 38% homology with the mammalian tachykinin NK3 receptor within the transmembrane domain region. Stable cell lines expressing this cDNA are responsive to Locusta migratoria tachykinin but not to other peptides of the tachykinin family. The expression of this gene is detected principally in adult fly heads, but also in the adult body and in embryos. Interestingly, NKD mRNA is detected at very early stages of Drosophila embryonic development (3 h) and reaches the highest level of expression at 12-16 h, a time which correlates with the period of major neuronal development. In situ hybridization experiments demonstrate that NKD is expressed in the central nervous system, as well as in subsets of neurons in each segment of the developing ventral ganglia. The cytological localization of this gene is at position 86C on the Drosophila third chromosome.
已描述了多种存在于昆虫神经系统中的神经肽。这些神经肽的生理作用尚未阐明。我们鉴定了一个果蝇cDNA，其编码一种名为NKD的蛋白质，该蛋白质的序列与哺乳动物G蛋白偶联神经肽受体的序列相似。该蛋白质在跨膜结构域区域与哺乳动物速激肽NK3受体具有38%的同源性。表达该cDNA的稳定细胞系对飞蝗速激肽有反应，但对速激肽家族的其他肽无反应。该基因的表达主要在成年果蝇头部检测到，但在成年果蝇身体和胚胎中也有检测到。有趣的是，在果蝇胚胎发育的早期阶段（3小时）就检测到了NKD mRNA，并且在12 - 16小时达到最高表达水平，这个时期与主要神经元发育时期相关。原位杂交实验表明，NKD在中枢神经系统以及发育中的腹神经节各节段的神经元亚群中表达。该基因的细胞学定位在果蝇第三条染色体的86C位置。
