Rosay P, Colas J F, Maroteaux L
Institut de Génétique, Université L. Pasteur de Strasbourg, CNRS, INSERM, France.
Mech Dev. 1995 Jun;51(2-3):329-39. doi: 10.1016/0925-4773(95)00382-7.
Neuropeptide function in the peripheral and central nervous systems has been described in mammals as well as in insects. We previously reported the cloning of the neuropeptide receptor NKD, a Drosophila melanogaster homologue of the mammalian tachykinin receptors. This receptor is expressed during Drosophila embryonic development and in the adult fly. Use of the NKD promoter region to drive beta-galactosidase expression in transgenic flies reveals a bipartite promoter organisation: the distal region controls NKD expression in neurosecretory cells of the central nervous system during late embryogenesis, whereas the proximal region is responsible for transient expression in peripheral nervous system during late embryogenesis, whereas the proximal region is responsible for transient expression in peripheral nervous system precursor cells early in development. This early NKD expression, first restricted to the sensory organ precursor cell, an atonal positive cell, is abolished in the ato1 mutant. In addition, we show that the proneural protein atonal, in association with daughterless, transactivates the NKD promoter in Schneider S2 cells via the proximal E box NKDE2. Furthermore, heterodimers of atonal and daughterless interact with this E box in gel shift assay.
神经肽在哺乳动物和昆虫的外周及中枢神经系统中的功能已被描述。我们之前报道了神经肽受体NKD的克隆，它是哺乳动物速激肽受体在黑腹果蝇中的同源物。该受体在果蝇胚胎发育期间及成年果蝇中均有表达。利用NKD启动子区域驱动转基因果蝇中的β-半乳糖苷酶表达，揭示了一种二分启动子结构：远端区域在胚胎发育后期控制中枢神经系统神经分泌细胞中的NKD表达，而近端区域在胚胎发育后期负责在外周神经系统中的瞬时表达，而近端区域在发育早期负责在外周神经系统前体细胞中的瞬时表达。这种早期的NKD表达最初局限于感觉器官前体细胞，一种无调性阳性细胞，在ato1突变体中消失。此外，我们表明，神经前体蛋白无调性与无女儿蛋白一起，通过近端E盒NKDE2在施耐德S2细胞中转激活NKD启动子。此外，在凝胶迁移试验中，无调性和无女儿蛋白的异二聚体与该E盒相互作用。
