Sato M, Sato T, Ose Y, Nagase H, Kito H, Sakai Y
Gifu Pharmaceutical University, Japan.
Mutat Res. 1992 Feb;265(2):149-54. doi: 10.1016/0027-5107(92)90043-2.
The modulating effects of the Chinese medicinal plant 'Tan-shen', the radix of Salvia miltiorrhiza Bunge, on the mutagenic activities of Trp-P-1 (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole) and B(a)P (benzo[a]pyrene) were investigated using Salmonella typhimurium TA98. Ether- and hot water-extracted 'Tan-shen' enhanced both mutagens at low concentrations, but suppressed them at high concentrations. Extracts by ether treatment were more effective than those extracted by hot water. Dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA were isolated from the ether extract by high performance liquid chromatography (HPLC) and were recognized to be the mutagenic modulators. 4 tanshinones enhanced the mutagenicity of Trp-P-1 by 8-24-fold at 20 micrograms/plate and the enhancement was reduced at the higher concentration. Dihydrotanshinone I suppressed Trp-P-1 activity completely at 100 micrograms/plate.
采用鼠伤寒沙门氏菌TA98研究了中药“丹参”（丹参的根）对Trp-P-1（3-氨基-1,4-二甲基-5H-吡啶并[4,3-b]吲哚）和B(a)P（苯并[a]芘）致突变活性的调节作用。乙醚提取物和热水提取物在低浓度时增强两种诱变剂的活性，但在高浓度时抑制它们。乙醚处理提取物比热水提取物更有效。通过高效液相色谱法（HPLC）从乙醚提取物中分离出二氢丹参酮I、隐丹参酮、丹参酮I和丹参酮IIA，它们被认为是诱变调节剂。4种丹参酮在20微克/平板时使Trp-P-1的致突变性增强8至24倍，在较高浓度时增强作用减弱。二氢丹参酮I在100微克/平板时完全抑制Trp-P-1的活性。
