大肠杆菌天然和衍生的30S及50S核糖体的比较。

A comparison of the native and derived 30S and 50S ribosomes of Escherichia coli.

作者信息

GREEN M H, HALL B D

出版信息

Biophys J. 1961 Jul;1(6):517-23. doi: 10.1016/s0006-3495(61)86905-1.

DOI:10.1016/s0006-3495(61)86905-1

PMID:13708199

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1366336/

Abstract

Four types of ribosomes occurring in E. coli have been separated by sucrose gradient centrifugation. These are the 30S and 50S particles occurring in E. coli extracts (native particles), and the 30S and 50S particles which are the subunits of 70S ribosomes (derived particles). Two criteria were used in comparing these particles: (1) The type of RNA contained in each, as determined by sedimentation velocity in the analytical ultracentrifuge. (2) The ability of mixtures of 30S and 50S ribosomes (derived 30S + derived 50S, native 30S + native 50S) to undergo the reaction: [Formula: see text] Native and derived 30S particles were found to contain 16S RNA. Derived 50S particles contained 23S RNA and a small amount of 15 to 20S RNA, whereas native 50S ribosomes contained only 16S RNA. Derived 30S and 50S particles combined to form 70S particles. However, under identical conditions, native 30S and 50S particles did not form 70S ribosomes.

摘要

通过蔗糖梯度离心法已分离出大肠杆菌中存在的四种核糖体。这些是大肠杆菌提取物中存在的30S和50S颗粒（天然颗粒），以及作为70S核糖体亚基的30S和50S颗粒（衍生颗粒）。比较这些颗粒时使用了两个标准：（1）通过分析超速离心机中的沉降速度确定每种颗粒所含的RNA类型。（2）30S和50S核糖体混合物（衍生30S +衍生50S，天然30S +天然50S）进行反应的能力：[公式：见正文] 发现天然和衍生的30S颗粒含有16S RNA。衍生的50S颗粒含有23S RNA和少量15至20S RNA，而天然50S核糖体仅含有16S RNA。衍生的30S和50S颗粒结合形成70S颗粒。然而，在相同条件下，天然30S和50S颗粒不会形成70S核糖体。