Santer M, Smith J R
J Bacteriol. 1966 Oct;92(4):1099-110. doi: 10.1128/jb.92.4.1099-1110.1966.
Santer, Melvin (Haverford College, Haverford, Pa.), and Josephine R. Smith. Ribonuclease sensitivity of Escherichia coli ribosomes. J. Bacteriol. 92:1099-1110. 1966.-The ribonucleic acid (RNA) contained in 70S ribosomes and in 50S and 30S subunits was hydrolyzed by pancreatic ribonuclease. A 7% amount of the RNA was removed from the 70S particle; at 10(-4)m magnesium concentration, a maximum of 24 and 30% of the RNA in the 50S and the 30S fractions, respectively, was removed by ribonuclease. At the two lower magnesium ion concentrations, 50S ribosomes did not lose any protein, whereas 30S ribosomes lost protein as a result of ribonuclease treatment. A number of proteins were removed from the 30S particles by ribonuclease, and these proteins were antigenically related to proteins present in 50S ribosomes. The differential effect of ribonuclease on 50S and 30S ribosomes suggested that they have structural dissimilarities.
桑特，梅尔文（哈弗福德学院，宾夕法尼亚州哈弗福德），以及约瑟芬·R·史密斯。大肠杆菌核糖体的核糖核酸酶敏感性。《细菌学杂志》92:1099 - 1110。1966年。——70S核糖体以及50S和30S亚基中所含的核糖核酸（RNA）被胰核糖核酸酶水解。从70S颗粒中去除了7%的RNA；在镁离子浓度为10^(-4)m时，核糖核酸酶分别从50S和30S组分中最多去除了24%和30%的RNA。在两个较低的镁离子浓度下，50S核糖体没有损失任何蛋白质，而30S核糖体在核糖核酸酶处理后损失了蛋白质。核糖核酸酶从30S颗粒中去除了一些蛋白质，这些蛋白质与50S核糖体中存在的蛋白质具有抗原相关性。核糖核酸酶对50S和30S核糖体的不同作用表明它们在结构上存在差异。
