Kamito T, Tanaka H, Sato B, Nagasawa H, Suzuki A
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1992 Jan 31;182(2):514-9. doi: 10.1016/0006-291x(92)91762-f.
The cDNAs encoding eclosion hormone (EH) of the silkworm, Bombyx mori, were isolated and sequenced. The results showed that the pre-EH molecule contains a 26-amino acid signal peptide and a 62-amino acid mature EH. The deduced amino acid sequence agreed with that previously determined by the peptide analysis. The presence of leucine residue at the carboxyl terminal of EH, which had not been detected directly by the peptide analysis, was proved. Primer extension and Northern hybridization analyses revealed that 0.9 kb mRNA is transcribed and it has a 66-nucleotide non-translated sequence at the 5'-end region. In situ hybridization showed that the EH gene is expressed in two pairs of nuerosecretory cells in the brain of 5th instar larva.
对家蚕编码羽化激素（EH）的cDNA进行了分离和测序。结果表明，前羽化激素分子包含一个26个氨基酸的信号肽和一个62个氨基酸的成熟羽化激素。推导的氨基酸序列与先前通过肽分析确定的序列一致。证实了在羽化激素羧基末端存在亮氨酸残基，这在肽分析中未直接检测到。引物延伸和Northern杂交分析表明，转录出了0.9 kb的mRNA，其5'端区域有一个66个核苷酸的非翻译序列。原位杂交显示，羽化激素基因在五龄幼虫大脑中的两对神经分泌细胞中表达。
