Differential susceptibility of phosphatidylcholine small unilamellar vesicles to phospholipases A2, C and D in the presence of membrane active peptides.

Activities of phospholipases C and D along with A2 were followed on egg phosphatidylcholine small unilamellar vesicles in the presence of membrane active peptides melittin, gramicidin S and alamethicin. Decrease in the activity of phospholipase C and D and enhancement of phospholipases A2 activity suggest that these enzymes are sensitive to alterations in the lipid packing in the membranes in the presence of these peptides. Phospholipase C and D, which have not been used to study peptide--membrane interactions, have potential use in studying membrane perturbations, since their activities are very sensitive to lipid packing.