环磷酸腺苷（cAMP）对激活素βA信使核糖核酸（mRNA）水平的调控

Regulation of activin beta A mRNA level by cAMP.

作者信息

Tanimoto K, Tamura K, Ueno N, Usuki S, Murakami K, Fukamizu A

机构信息

Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.

出版信息

Biochem Biophys Res Commun. 1992 Jan 31;182(2):773-8. doi: 10.1016/0006-291x(92)91799-v.

DOI:10.1016/0006-291x(92)91799-v

PMID:1370892

Abstract

We demonstrated the presence of five species of the activin beta A mRNA in human placenta and one major RNA associated with two minor RNAs of the activin in the fetal membrane. We investigated the effect of 8-bromo-cAMP (8-Br-cAMP) on accumulation of activin beta A subunit mRNA in human fibrosarcoma HT1080 cells. Although low levels of the activin mRNA were detectable in the untreated cells, the one main RNA species was predominantly accumulated by 8-Br-cAMP. We propose that generation of multiple activin mRNAs in the fetal membrane and cAMP-treated HT1080 cells is presumably due to a cell-specific alternative polyadenylation.

摘要

我们证实在人胎盘中存在5种激活素βA信使核糖核酸（mRNA），且在胎膜中有1种主要RNA与激活素的2种次要RNA相关联。我们研究了8-溴环磷酸腺苷（8-Br-cAMP）对人纤维肉瘤HT1080细胞中激活素βA亚基mRNA积累的影响。虽然在未处理的细胞中可检测到低水平的激活素mRNA，但8-Br-cAMP主要使1种主要RNA种类积累。我们提出，胎膜和经cAMP处理的HT1080细胞中多种激活素mRNA的产生可能是由于细胞特异性可变聚腺苷酸化。