Willems A, Goor M, Thielemans S, Gillis M, Kersters K, De Ley J
Laboratorium voor Microbiologie en microbiële Genetica, Rijksuniversiteit Gent, Belgium.
Int J Syst Bacteriol. 1992 Jan;42(1):107-19. doi: 10.1099/00207713-42-1-107.
DNA-rRNA hybridizations, DNA-DNA hybridizations, polyacrylamide gel electrophoresis of whole-cell proteins, and a numerical analysis of carbon assimilation tests were carried out to determine the relationships among the phylogenetically misnamed phytopathogenic taxa Pseudomonas avenae, Pseudomonas rubrilineans, "Pseudomonas setariae," Pseudomonas cattleyae, Pseudomonas pseudoalcaligenes subsp. citrulli, and Pseudomonas pseudoalcaligenes subsp. konjaci. These organisms are all members of the family Comamonadaceae, within which they constitute a separate rRNA branch. Only P. pseudoalcaligenes subsp. konjaci is situated on the lower part of this rRNA branch; all of the other taxa cluster very closely around the type strain of P. avenae. When they are compared phenotypically, all of the members of this rRNA branch can be differentiated from each other, and they are, as a group, most closely related to the genus Acidovorax. DNA-DNA hybridization experiments showed that these organisms constitute two genotypic groups. We propose that the generically misnamed phytopathogenic Pseudomonas species should be transferred to the genus Acidovorax as Acidovorax avenae and Acidovorax konjaci. Within Acidovorax avenae we distinguished the following three subspecies: Acidovorax avenae subsp. avenae, Acidovorax avenae subsp. cattleyae, and Acidovorax avenae subsp. citrulli. Emended descriptions of the new taxa are presented.
进行了DNA-rRNA杂交、DNA-DNA杂交、全细胞蛋白的聚丙烯酰胺凝胶电泳以及碳同化试验的数值分析，以确定在系统发育上命名错误的植物病原分类单元燕麦假单胞菌、红条假单胞菌、“狗尾草假单胞菌”、卡特兰假单胞菌、西瓜嗜麦芽窄食单胞菌亚种和魔芋嗜麦芽窄食单胞菌亚种之间的关系。这些生物都是丛毛单胞菌科的成员，在该科中它们构成一个单独的rRNA分支。只有魔芋嗜麦芽窄食单胞菌位于这个rRNA分支的下部；所有其他分类单元都非常紧密地聚集在燕麦假单胞菌模式菌株周围。当对它们进行表型比较时，这个rRNA分支的所有成员都可以相互区分，并且作为一个群体，它们与嗜酸菌属关系最为密切。DNA-DNA杂交实验表明这些生物构成两个基因型组。我们建议将在属的分类上命名错误的植物病原假单胞菌物种转移到嗜酸菌属，分别为燕麦嗜酸菌和魔芋嗜酸菌。在燕麦嗜酸菌内，我们区分出以下三个亚种：燕麦嗜酸菌燕麦亚种、燕麦嗜酸菌卡特兰亚种和燕麦嗜酸菌西瓜亚种。本文给出了新分类单元的修订描述。
