Fedi A M, Lotti T, Meo A L, Rizzo A, Leoncini P
Department of Dermatology I, University of Florence, Italy.
Int J Dermatol. 1992 Jan;31(1):30-2. doi: 10.1111/j.1365-4362.1992.tb03516.x.
Three biopsies of normal skin and 15 biopsies collected from patients with psoriasis vulgaris were analyzed for the expression of the 50 kd cytokeratin using direct immunofluorescence and ABC technique before and after local treatment with anthralin 0.1% in a petrolatum base, with 0.05% betamethasone dipropionate cream, and finally, after PUVA treatment. Antiserum against the 50 kd anti-cytokeratin reacted with tissue sections of normal skin, staining cells in the basal layer, while the psoriatic skin sections before the various treatments showed a staining concerning the whole thickness of the epidermis. After the various therapies, the 50 kd cytokeratin immunoreactivity was observed only in the basal layer of those psoriatic skin sections that showed complete clinical clearing, while it was observed in the whole thickness of psoriatic patches that did not clear. These data suggest that the normalization of the 50 kd cytokeratin expression pattern can be considered as a marker of clinical remission of psoriasis.
采用直接免疫荧光法和ABC技术，对3份正常皮肤活检标本以及15份寻常型银屑病患者的活检标本进行分析，检测50kd细胞角蛋白的表达情况。这些标本分别在使用0.1%蒽林凡士林基质、0.05%丙酸倍他米松乳膏局部治疗前、治疗后，以及最终接受光化学疗法（PUVA）治疗后进行检测。抗50kd抗细胞角蛋白抗血清与正常皮肤组织切片发生反应，使基底层细胞着色，而在各种治疗前的银屑病皮肤切片中，整个表皮厚度均显示有染色。经过各种治疗后，仅在那些临床完全清除的银屑病皮肤切片的基底层观察到50kd细胞角蛋白免疫反应性，而在未清除的银屑病斑块全层均观察到该反应性。这些数据表明，50kd细胞角蛋白表达模式的正常化可被视为银屑病临床缓解的一个标志物。
