Fedi A M, Leoncini P, Farella V, Lotti T
G Ital Dermatol Venereol. 1989 Apr;124(4):141-5.
Filaggrin is a histidine-rich basic protein that aggregates keratin filaments in fully differentiated cells of the epidermis. Filaggrin is synthesized in the granular cell layer as a high-Mr phosphorylated precursor, profilaggrin, that is processed to form the lower-Mr product present in cornified cells. The catabolism of filaggrin in stratum corneum produces urocanic acid and carboxylic-pyrrolidone acid that, respectively, absorb UV radiations and support cutaneous hydratation. In this study we evaluated by direct-immunofluorescence and by immunoperoxidase staining using rabbit antihuman filaggrin antiserum localization of filaggrin in psoriatic skin and in normal human skin before and after treatment with anthralin 0.1%, betamethasone 0.05% and hydrocolloid dressing. Antiserum against human filaggrin reacted with tissue sections of normal human skin, staining cells in the granular layer and in the stratum corneum, while no staining of human psoriatic skin sections was observed. After treatments, filaggrin resulted present in those psoriatic skin sections that showed complete clinical remission, while it was not observed in psoriatic patches which did not clear. These studies suggest that human skin filaggrin can be considered a marker of clinical remission of psoriasis.
丝聚合蛋白是一种富含组氨酸的碱性蛋白，可在表皮完全分化的细胞中使角蛋白丝聚集。丝聚合蛋白在颗粒细胞层中作为一种高分子量的磷酸化前体——前丝聚合蛋白合成，该前体经过加工形成存在于角质化细胞中的低分子量产物。角质层中丝聚合蛋白的分解代谢产生尿刊酸和羧基吡咯烷酮酸，它们分别吸收紫外线并支持皮肤水合作用。在本研究中，我们通过直接免疫荧光法以及使用兔抗人丝聚合蛋白抗血清的免疫过氧化物酶染色法，评估了丝聚合蛋白在银屑病皮肤以及用0.1%蒽林、0.05%倍他米松和水胶体敷料治疗前后的正常人皮肤中的定位情况。抗人丝聚合蛋白抗血清与正常人皮肤组织切片发生反应，对颗粒层和角质层中的细胞进行染色，而未观察到对人银屑病皮肤切片的染色。治疗后，丝聚合蛋白出现在那些显示出完全临床缓解的银屑病皮肤切片中，而在未清除的银屑病斑块中未观察到。这些研究表明，人皮肤丝聚合蛋白可被视为银屑病临床缓解的一个标志物。
